| № | # | Title | Directed by | Written by | Original air date | Production
code | U.S. viewers
(million) |
| 36 | 1 | "Chuck Versus the Pink Slip" | Robert Duncan McNeill | Chris Fedak & Matt Miller | January 10, 2010 (NBC) | 3X5801 | 7.703 |
| 37 | 2 | "Chuck Versus the Three Words" | Peter Lauer | Allison Adler & Scott Rosenbaum | January 10, 2010 (NBC) | 3X5802 | 7.198 |
| 38 | 3 | "Chuck Versus the Angel de la Muerte" | Jeremiah Chechik | Phil Klemmer | January 11, 2010 (NBC) | 3X5803 | 7.355 |
| 39 | 4 | "Chuck Versus Operation Awesome" | Robert Duncan McNeill | Zev Borow | January 17, 2010 (Citytv) | 3X5804 | 6.650 |
| 40 | 5 | "Chuck Versus First Class" | Fred Toye | Chris Fedak | January 24, 2010 (Citytv) | 3X5805 | 6.977 |
| 41 | 6 | "Chuck Versus the Nacho Sampler" | Allan Kroeker | Matt Miller & Scott Rosenbaum | January 31, 2010 (Citytv) | 3X5806 | 6.732 |
| 42 | 7 | "Chuck Versus the Mask" | Michael Schultz | Phil Klemmer | February 8, 2010 (NBC) | 3X5807 | 6.596 |
| 43 | 8 | "Chuck Versus the Fake Name" | Jeremiah Chechik | Allison Adler | March 1, 2010 (NBC) | 3X5808 | 6.701 |
| 44 | 9 | "Chuck Versus the Beard" | Zachary Levi | Scott Rosenbaum | March 8, 2010 (NBC) | 3X5809 | 6.367 |
| 45 | 10 | "Chuck Versus the Tic Tac" | Patrick Norris | Rafe Judkins & Lauren LeFranc | March 15, 2010 (NBC) | 3X5810 | 5.849 |
| 46 | 11 | "Chuck Versus the Final Exam" | Robert Duncan McNeill | Zev Borow | March 22, 2010 (NBC) | 3X5811 | 5.461 |
| 47 | 12 | "Chuck Versus the American Hero" | Jeremiah Chechik | Story by: Max Denby
Teleplay by: Matt Miller & Phil Klemmer | March 29, 2010 (NBC) | 3X5812 | 5.679 |
| 48 | 13 | "Chuck Versus the Other Guy" | Peter Lauer | Chris Fedak | April 5, 2010 (NBC) | 3X5813 | 5.793 |
| 49 | 14 | "Chuck Versus the Honeymooners" | Robert Duncan McNeill | Story by: Allison Adler
Teleplay by: Rafe Judkins & Lauren LeFranc | April 26, 2010 (NBC) | 3X5814 | 5.783 |
| 50 | 15 | "Chuck Versus the Role Models" | Fred Toye | Phil Klemmer | May 3, 2010 (NBC) | 3X5815 | 5.345 |
| 51 | 16 | "Chuck Versus the Tooth" | Daisy von Scherler Mayer | Zev Borow & Max Denby | May 10, 2010 (NBC) | 3X5816 | 5.330 |
| 52 | 17 | "Chuck Versus the Living Dead" | Jay Chandrasekhar | Lauren LeFranc & Rafe Judkins | May 17, 2010 (NBC) | 3X5817 | 5.200 |
| 53 | 18 | "Chuck Versus the Subway" | Matt Shakman | Story by: Matt Miller
Teleplay by: Allison Adler & Phil Klemmer | May 24, 2010 (NBC) | 3X5818 | 4.960 |
| 54 | 19 | "Chuck Versus the Ring: Part II" | Robert Duncan McNeill | Josh Schwartz & Chris Fedak | May 24, 2010 (NBC) | 3X5819 | 5.160 |
